Characterization of new variants of avian infectious bronchitis virus in Tunisia.
Identifieur interne : 000094 ( Main/Exploration ); précédent : 000093; suivant : 000095Characterization of new variants of avian infectious bronchitis virus in Tunisia.
Auteurs : Hager Bourogâa [Tunisie] ; Khaled Miled ; Latifa Gribâa ; Imen El Behi ; Abdeljelil GhramSource :
- Avian diseases [ 0005-2086 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Génotype (MeSH), Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (virologie), Infections à coronavirus (épidémiologie), Maladies de la volaille (virologie), Maladies de la volaille (épidémiologie), Phylogenèse (MeSH), Poulets (MeSH), Protéines de capside (génétique), Protéines de capside (métabolisme), Régulation de l'expression des gènes viraux (physiologie), Séquence d'acides aminés (MeSH), Tunisie (épidémiologie), Variation génétique (MeSH), Virus de la bronchite infectieuse (génétique).
- MESH :
- génétique : Protéines de capside, Virus de la bronchite infectieuse.
- médecine vétérinaire : Infections à coronavirus.
- métabolisme : Protéines de capside.
- physiologie : Régulation de l'expression des gènes viraux.
- virologie : Infections à coronavirus, Maladies de la volaille.
- épidémiologie : Infections à coronavirus, Maladies de la volaille, Tunisie.
- Animaux, Génotype, Phylogenèse, Poulets, Séquence d'acides aminés, Variation génétique.
- Wicri :
- geographic : Tunisie.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Animals (MeSH), Capsid Proteins (genetics), Capsid Proteins (metabolism), Chickens (MeSH), Coronavirus Infections (epidemiology), Coronavirus Infections (veterinary), Coronavirus Infections (virology), Gene Expression Regulation, Viral (physiology), Genetic Variation (MeSH), Genotype (MeSH), Infectious bronchitis virus (genetics), Phylogeny (MeSH), Poultry Diseases (epidemiology), Poultry Diseases (virology), Tunisia (epidemiology).
- MESH :
- chemical , genetics : Capsid Proteins.
- chemical , metabolism : Capsid Proteins.
- geographic , epidemiology : Tunisia.
- epidemiology : Coronavirus Infections, Poultry Diseases.
- genetics : Infectious bronchitis virus.
- physiology : Gene Expression Regulation, Viral.
- veterinary : Coronavirus Infections.
- virology : Coronavirus Infections, Poultry Diseases.
- Amino Acid Sequence, Animals, Chickens, Genetic Variation, Genotype, Phylogeny.
Abstract
Three infectious bronchitis virus (IBV) strains, isolated from suspected Tunisian broiler flocks, were characterized as variant viruses using genotyping and serotyping techniques. They were compared with commonly used vaccine strains, including 793/B, D274, and Massachusetts types. Reverse transcription-PCR-restriction fragment length polymorphism, nucleotide sequencing, and GenBank BLAST database analyses of the hypervariable region of the S1 subunit of the virus spike gene showed that the three isolates, designated TN20/00, TN200/01, and TN335/01, share from 64% to 82% homologies between each other but are very different from the H120 strain, the only infectious bronchitis vaccine used in Tunisia. In addition, they showed from 57% to 78% similarities with the European genotypes, including D274 and 793/B. Phylogenetic data allowed classification of the three Tunisian isolates as new genotypes placed inside the same genetic group as the CR88121 and D274 genotypes but very distant from the Massachusetts genotype. Cross-virus neutralization tests confirmed the genotyping results and showed that both TN200/01 and TN335/01 isolates are serologically related, whereas the TN20/00 is closer to TN335/01 than to TN200/01. Moreover, all three Tunisian isolates are closely related to the European variant serotypes, including the CR88121 and the D274 strains, but none is serologically related to the H120 vaccine strain. These data demonstrated, for the first time in Tunisia, the cocirculation of IBV variant serotypes along with the Massachusetts type, causing severe clinical diseases and high economic losses to the poultry industry.
DOI: 10.1637/8666-022609-Reg.1
PubMed: 19848084
Affiliations:
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Le document en format XML
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Gribâa</name></author><author><name sortKey="El Behi, Imen" sort="El Behi, Imen" uniqKey="El Behi I" first="Imen" last="El Behi">Imen El Behi</name></author><author><name sortKey="Ghram, Abdeljelil" sort="Ghram, Abdeljelil" uniqKey="Ghram A" first="Abdeljelil" last="Ghram">Abdeljelil Ghram</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19848084</idno><idno type="pmid">19848084</idno><idno type="doi">10.1637/8666-022609-Reg.1</idno><idno type="wicri:Area/PubMed/Corpus">000725</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000725</idno><idno type="wicri:Area/PubMed/Curation">000721</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000721</idno><idno type="wicri:Area/PubMed/Checkpoint">000744</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000744</idno><idno 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(veterinary)</term><term>Coronavirus Infections (virology)</term><term>Gene Expression Regulation, Viral (physiology)</term><term>Genetic Variation (MeSH)</term><term>Genotype (MeSH)</term><term>Infectious bronchitis virus (genetics)</term><term>Phylogeny (MeSH)</term><term>Poultry Diseases (epidemiology)</term><term>Poultry Diseases (virology)</term><term>Tunisia (epidemiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Génotype (MeSH)</term><term>Infections à coronavirus (médecine vétérinaire)</term><term>Infections à coronavirus (virologie)</term><term>Infections à coronavirus (épidémiologie)</term><term>Maladies de la volaille (virologie)</term><term>Maladies de la volaille (épidémiologie)</term><term>Phylogenèse (MeSH)</term><term>Poulets (MeSH)</term><term>Protéines de capside (génétique)</term><term>Protéines de capside (métabolisme)</term><term>Régulation de l'expression des gènes viraux (physiologie)</term><term>Séquence d'acides aminés (MeSH)</term><term>Tunisie (épidémiologie)</term><term>Variation génétique (MeSH)</term><term>Virus de la bronchite infectieuse (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Capsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Capsid Proteins</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Tunisia</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Coronavirus Infections</term><term>Poultry Diseases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de capside</term><term>Virus de la bronchite infectieuse</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à 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Sequence</term><term>Animals</term><term>Chickens</term><term>Genetic Variation</term><term>Genotype</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Génotype</term><term>Phylogenèse</term><term>Poulets</term><term>Séquence d'acides aminés</term><term>Variation génétique</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Tunisie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Three infectious bronchitis virus (IBV) strains, isolated from suspected Tunisian broiler flocks, were characterized as variant viruses using genotyping and serotyping techniques. They were compared with commonly used vaccine strains, including 793/B, D274, and Massachusetts types. Reverse transcription-PCR-restriction fragment length polymorphism, nucleotide sequencing, and GenBank BLAST database analyses of the hypervariable region of the S1 subunit of the virus spike gene showed that the three isolates, designated TN20/00, TN200/01, and TN335/01, share from 64% to 82% homologies between each other but are very different from the H120 strain, the only infectious bronchitis vaccine used in Tunisia. In addition, they showed from 57% to 78% similarities with the European genotypes, including D274 and 793/B. Phylogenetic data allowed classification of the three Tunisian isolates as new genotypes placed inside the same genetic group as the CR88121 and D274 genotypes but very distant from the Massachusetts genotype. Cross-virus neutralization tests confirmed the genotyping results and showed that both TN200/01 and TN335/01 isolates are serologically related, whereas the TN20/00 is closer to TN335/01 than to TN200/01. Moreover, all three Tunisian isolates are closely related to the European variant serotypes, including the CR88121 and the D274 strains, but none is serologically related to the H120 vaccine strain. These data demonstrated, for the first time in Tunisia, the cocirculation of IBV variant serotypes along with the Massachusetts type, causing severe clinical diseases and high economic losses to the poultry industry.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="El Behi, Imen" sort="El Behi, Imen" uniqKey="El Behi I" first="Imen" last="El Behi">Imen El Behi</name><name sortKey="Ghram, Abdeljelil" sort="Ghram, Abdeljelil" uniqKey="Ghram A" first="Abdeljelil" last="Ghram">Abdeljelil Ghram</name><name sortKey="Gribaa, Latifa" sort="Gribaa, Latifa" uniqKey="Gribaa L" first="Latifa" last="Gribâa">Latifa Gribâa</name><name sortKey="Miled, Khaled" sort="Miled, Khaled" uniqKey="Miled K" first="Khaled" last="Miled">Khaled Miled</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Bourogaa, Hager" sort="Bourogaa, Hager" uniqKey="Bourogaa H" first="Hager" last="Bourogâa">Hager Bourogâa</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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